METEOR

Reduce transfer steps and improve sample yield with integrated cryo-CLEM

METEOR overcomes the challenges in the current cryogenic electron tomography (cryo-ET) workflow by providing the capability to perform in situ fluorescence light microscopy (FLM) in your cryo focused ion beam (FIB)/ scanning electron microscope (SEM) chamber. Not only does it reduce the number of transfer steps between microscopes, protecting the fragile sample from unnecessary contamination, it will also allow you to confirm the presence of the region of interest (ROI) in the lamella directly after FIB milling.

Introduction

METEOR is highly adaptable to your current workflow and works well with your transfer systems and sample holders, making it easy to adopt.
METEOR can ensure an efficient workflow and increased sample yield for downstream acquisition of high resolution three dimensional biological structures in their near-native cellular environment. It’s a powerful tool that can be applied to a wide range of research areas including microbiology, neurosciences, immunology, virology, developmental, cell and molecular biology.

Benefits

Integration minimizes transfer steps
By integrating an FLM into your FIB/SEM many transfer steps are removed, reducing the risk of damage, devitrification or contamination of your sample.

Increase yield
Currently, the yield of the cryo-CLEM workflow is as low as 20%, METEOR will increase the yield by minimizing complicated transfer steps.

Increase throughput of samples
By simplifying the workflow METEOR will increase the throughput of the sample preparation for cryo-ET and ultimately allows for a higher efficiency in the analysis of biological structures to be achieved.

Less sample damage
Less sample damage translates into less cryo-TEM time being spent on substandard samples, resulting in a lower cost per sample analysed.

Reduce costs
Reduces purchase and maintenance costs by abolishing the need for a separate cryo- FLM system.

Key features

Improve cost efficiencies
Use your cryo TEM time more effectively on useful lamellae. Remove the need for a separate cryo FLM dedicated solely to ROI finding.

Optimize your workflow
Save time and work more efficiently by having both FLM and FIB in the same microscope.

Boost productivity
Produce high quality lamellae more easily. Obtain insight into your biological system through getting useful tomography data more quickly.

Increase sample yield
Target your ROIs and correlate better and more effectively with the integrated FLM. Reduce transfer steps and thereby sample damage.

Workflow at a glance

System specifications

Optics

Objectives: the following options are available*

    Olympus Fluorite objective

  • 10x (WD 11.0 mm, NA 0.30)
  • 20x (WD 3.1 mm, NA 0.45)
  • 50x (WD 1.0 mm, NA 0.80)
  • 100x (WD 1.0 mm, NA 0.90)
    • Olympus Apochromat objective

  • 50x (WD 0.35 mm, NA 0.95)
  • 100x (WD 0.35 mm, NA 0.95)
  • * Depending on shuttle and sample stage configuration the choice of objectives can be limited due to space restrictions.

    Lightsource:

    Omicron LedHub fitted with 4 LED sources:

  • 385 nm
  • 470 nm
  • 505 – 600 nm – bandpass filter
  • 625 nm
  • Camera :

    1.31 MPix 10bit CMOS camera (UI-5240SE Rev. 4)

    Filters :

    Equipped with filter wheel (can be adjusted according to the clients wishes):

  • 440/40 nm single-band bandpass filter
  • 525/30 nm single-band bandpass filter
  • 607/36 nm single-band bandpass filter.
  • 684/24 nm single-band bandpass filter.
  • 410/504/582/669 nm dichroic beamsplitter.
  • System Control

    Comes with a control PC for acquiring your fluorescence images

    Odemis Integrated Software

    Open source software suite