DELPHI

The world’s first fully integrated SEM and fluorescence microscope

By adding SEM contrast to your fluorescence imaging, structural and functional details can be obtained in a single image.

The integrated design makes the system easy to operate and extremely fast thanks to seamless switching between imaging techniques. The system also has the advantage of size and may be placed in any laboratory without the need for extra infrastructure.

Precision mechanics and the use of a proprietary automated alignment technique using the electron beam allows extremely precise, user-independent overlay of images with an accuracy of sub-50 nm.

The Delphi is the ultimate bridge between the fluorescence and electron microscopy world.

At a glance

  • Fully integrated, both in hardware and software
  • Easy to use and fast workflow, from sample loading to correlative imaging in less than a minute
  • Automated overlay using proprietary algorithm leading to perfect and unbiased overlays
  • No facilities required for installation

Applications

Typical application areas of the Delphi include whole cells cultured on cover-glass, as well as thin sections of cells, tissue or other biological material.

Example application showing human umbilical vein endothelial cells stained for nucleus, actin and mitochondria, overlaid with high resolution SEM data.


Specifications

Resolution
  • Electron microscope resolution is 20 nm
  • Fluorescence microscope resolution is 290 nm
General
  • Sample loading in less than a minute
  • Full color navigation camera 20 – 120x magnification
  • Power usage 700 W at maximum power
  • Footprint of 35*60*65 cm (w*d*h), excluding screens and pump
Electron optics
  • Magnification range 20 – 100,000x
  • Long-lifetime high-brightness source (CeB6)
  • Acceleration voltage from 4.8 kV to 10 kV
Objective lens
  • 40x, 0.95NA, Plan Apo objective lens
Fluorescence
  • Multiband setup in Pinkel configuration optimized for DAPI, FITC, TRITC, Cy5 and other like fluorophores
  • Four channel solid-state (LED) excitation source with digital On/Off and intensity control. Excitation at 395/485/575/650 nm
Camera
  • sCMOS camera with 2048 x 2048 pixels, with 6.5 μm pixels
  • Peak QE 60% at 600 nm with at least 40% QE over the range 420 nm to 800 nm