[custom_headline type=”left” level=”h1″ looks_like=”h2″]Ultramicrotome KATANA[/custom_headline]

A portable system for serial block-face SEM

Avec le système de Serial Block-face Katana, le microtome est installé sur la platine motorisée du MEB de manière non invasive.
Le couteau diamant vibrant surface le block échantillon en retirant des couches aussi minces que 15 nm.
Après chaque coupe, la surface du bloc exposé est imagée par un détecteur BSE. Cette méthode automatisée In Situ permet d’acquérir une série d’images électroniques sur un grand volume d’échantillon.

Portable between SEMs
  • Can select SEM according todataset requirements, cost, availability.
  • Quick and easy to switch between SEM and Serial Block-Face Imaging.

Unique oscillator design for improved cutting

  • Large oscillation with real-time feedback, tuneable from 0 to 100 nm.
  • Variable frequency, tuneable from 0 to 100 kHz.
  • Auto-resonance mode, allows lowest heat dissipation.

Debris mitigation

Raised imaging position (by up to 1mm) for shorter working distance.

Open source command set.

  • Simple serial connection allows control from other hardware.

Software supplied for automation on older SEMs.

  • Requires SEM PC with Win95 or higher.

¹ SBEMimage integration currently in alpha phase of development. SBEMimage: Versatile Acquisition Control Software for Serial Block-Face Electron Microscopy [Benjamin Titze, Christel
Genoud, and Rainer W. Friedrich].

² Maximum possible size that can be cut in one go. For ease of setup, knife positioning, and block shape considerations, a maximum of 1.2 x 1.2 x 1.2 (mm) is recommended.


[video src="https://video.wixstatic.com/video/4fa453_2e3bc40609f148fd95006d0c204b0002/1080p/mp4/file.mp4" /]

Mouse brain

[video src="https://video.wixstatic.com/video/4fa453_4ecfae49626446a0822ad16d4618dceb/1080p/mp4/file.mp4" /]

Lamprey spinal cord

[video src="https://video.wixstatic.com/video/4fa453_8e30104a13764125baf2dac775fd0b6f/720p/mp4/file.mp4" /]

Squid skin

Technical specifications

  • Microtome
  • Dimension : 135 x 66 x 56 (H) mm

    Z resolution : 1 nm (closed-loop)

    Z travel range : 1300 μm

    Knife travel range : 4 mm

    Diamond blade width : 1.5 mm

    Knife oscillation amplitude : 0 – 170 nm

    Knife oscillation frequency : 0 – 100 kHz

    Maximum sample size : 1.5 x 1.5 x 1.3 (X,Y,Z mm)

  • Accessories
  • Microtome | Viewer |  Measurement tool | Controller

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