TEM carbon support films have a hydrophobic tendency.
A glow discharge treatment with air makes the carbon film surface negatively charged and hydrophilic which allows an adsorption of aqueous solutions.

Thanks to a glow discharge treatment with amylamine the hydrophobic tendency of the carbon film is kept and the surface is charged positively allowing an adsorption of molecules like nucleic acids.

WITHOUT GLOW DISCHARGE

WITH GLOW DISCHARGE

Figure 1 : Negative staining with uranyl acetate of the viral capsids spread on a TEM grid without treatment.

Courtesy : M. Decossas, CBMN

Figure 2 : Negative staining with uranyl acetate of the Poliomyelite virus spread on a TEM grid with a glow discharge treatment with air (2mA during 40sec).

Courtesy : M. Decossas, CBMN

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EASY

• Quick and easy loading of films or grids.
• Intuitive operation with manual or programmed mode.
• Affichage (courant, pression, durée) en temps réel des données sur écrans LCD rétrReal-time display of process parameters (current, vacuum, time).

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FLEXIBLE

• Glow discharge methods : hydrophilic or hydrophobic,negative or positive charge.
• Dual vacuum Bell Jars to avoid cross contamination.
• Anti-implosion glass for an easy cleaning and a high secured process.

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REPRODUCIBLE

• Accurate injection control of gaz or liquids.
• Accurate vacuum control using Pirani gauge.
• Soft venting (air or neutral gaz) using quick inlet fitting.

Figure 3 : Negative staining with uranyl acetate and rotary shadowing with platinium of plasmid DNA.

Courtesy : C. Ruhlmann, IGBMC

Figure 4 : Negative staining with uranyl acetate and rotary shadowing with platinium of yeast chromatin.

Courtesy : C. Ruhlmann, IGBMC

Figure 5 : Negative staining with phosphotungstic acid of Influenza virus hemagglutinin.

Courtesy: M. Decossas, CBMN

Figure 6 : Negative staining with uranyl acetate of liposome and proteo-liposome.

Courtesy : L. Daury-Joucla, CBMN

Figure 7 : Torus obtained by complex coacervation between the peptide P140 and hyaluronic acid (therapeutic aim).

Courtesy : C. Blanck, ICS