The Most Cost Effective, Compact, User Friendly, Cryosectioning System Available.
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- Four user-adjustable temperature programs
- Efficient cryogenic design with lowest LN2 consumption
- Quick set-up and conversion back to room temperature ultramicrotomy
- Nine liter Dewar fits on table-top, yet provides LN2 for a whole day’s use
- Compatible with most modern ultramicrotomes
- Precision microprocessor temperature control from +35°C to -180°C
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The Golden Standard
With over 100 installations world-wide, and more than 400 scientific publications to its credit, the HPM-010 is not only the pioneer of HP freezing, but the instrument of choice which allows freezing of aqueous samples up to 200µm thickness without visible ice crystal damage and without the use of cryoprotectants.[/text_output]
The High Pressure Freezing Method (HPF)
High pressure freezing is the only method, that allows vitrification of samples up to 1mm3. The HPF method uses LN2 under high pressure (2100 bar). At 2100 bar, water is 1500 times more viscous than at atmospheric pressure, which drastically reduces the crystal growth.[/text_output]
Let’s compare!
Comparison of chemically fixed and high pressure frozen (using HPM 010) paramecium cells
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Regular distribution of Ribosomes in the Cytoplasm, granu- lar Glykogen is scarcely visible. Trichocystenbodies (TK) and Trichocystentips (TS) exhibit shrinkage. Surface membranes (M) are crinkled.
All Micrograph Photos: Dr. Heinrich Hohenberg, ETHZ Zurich, Lab. For EM1, CH – 8902 Zurich, Switzerland[/text_output]
Entire Paramecium cell is adequately cryopreserved. Glykogranula (GLY) has high density and occurs in delimited areas, as well as the Ribosomes (RI). Surface membranes are smooth and tight arranged.[/text_output]
Trichocyst bodies (TB) is detatched all round from the matrix. Glykogengranula is mixed with the Ribosomes.[/text_output]
No discernible shrinkage of the Trichocysts. Bodies and tips are firmly connected. Glykogengranula (GLY) is delimited from the Ribosome-areas (RI).[/text_output]
More Features…
- Cryo-fixation of suspensions, monolayer cell cultures and tissues
- Large base of application know-how available
- Shortest handling time before freezing
- Suited for suspension and tissue samples
- Easy one button operation
- In situ real time measurement of temperature and pressure
- Fast 90 second process cycle allows expeditious application
- Automatic, microprocessor controlled operation for routine work
- Suited for suspension and tissue samples
- Compact, sturdy unit with soundproof and vibration-free housing
- Simple and safe operation due to quick-locking action of specimen holder and clearly arranged operational controls
- Processing data is recorded on digital display, such as the actual temperature, time and pressure, thus allowing the user to exactly evaluate current operational status (sample quality control)
- Simple maintenance with removable cover plates and rack system for control units
- Extensive accessory program
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Dimensions and Weight
| Dimensions | see diagram below |
| Weight, approx. | 450 kg |
Working Data
| Working pressure | 2300 – 2600 bar |
| Maximum pressure | 2800 bar |
| Duration of working pressure, at least | 500 ms |
| Cooling time from 0°C to -50°C (measured between 3 mm copper disc) |
10 ms |
Specimen Dimensions
| Sample size, up to max. of | 200μm thickness and 2mm diameter |
Connection Data
| Voltages, frequencies | 3 x 380/220 V, 50 Hz or 3 x 208 V, 60 Hz |
| Power input, approx. | 23kVA |
| Compressed air (5 bar) | G 1/4” (outer thread |
| LN2 (1 bar excess pressure) | G 1/4” (outer thread) |
| Heating water (rubber hose) | Ø 7/14 mm |
Operational Data
| Hydraulic oil reservoir | 40 liters |
| Hydraulic oil bias pressure | 140 – 250 bar |
| LN2 Dewar (in system) | 7 liters |
| LN2 consumption | 10 – 20 liters/hour |
| Initial system cooling, approx. | 15 min. |
| Max. processing sequence, approx. | 40 shots/hour |
| lsopropyl alcohol reservoir, approx. | 0.5 liters |
[/text_output][image type=”none” float=”none” info=”none” info_place=”top” info_trigger=”hover” src=”2125″][text_output]Measurements in Millimeters[/text_output]
